De Novo Transcriptome Assembly of Isatis indigotica at Reproductive Stages and Identification of Candidate Genes Associated with Flowering Pathways

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High-Throughput Sequencing and De Novo Assembly of the Isatis indigotica Transcriptome

BACKGROUND Isatis indigotica, the source of the traditional Chinese medicine Radix isatidis (Ban-Lan-Gen), is an extremely important economical crop in China. To facilitate biological, biochemical and molecular research on the medicinal chemicals in I. indigotica, here we report the first I. indigotica transcriptome generated by RNA sequencing (RNA-seq). RESULTS RNA-seq library was created us...

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clustering of short read sequences for de novo transcriptome assembly

given the importance of transcriptome analysis in various biological studies and considering thevast amount of whole transcriptome sequencing data, it seems necessary to develop analgorithm to assemble transcriptome data. in this study we propose an algorithm fortranscriptome assembly in the absence of a reference genome. first, the contiguous sequencesare generated using de bruijn graph with d...

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ژورنال

عنوان ژورنال: Journal of the American Society for Horticultural Science

سال: 2018

ISSN: 0003-1062,2327-9788

DOI: 10.21273/jashs04232-17